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Medical University - Varna
Prof. Dr. Paraskev Stoyanov
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Assoc. Prof. Oskan Bahidinov Tasinov

84 Tzar Osvoboditel blvd., 9000 Varna

Department of Biochemistry, Molecular Medicine and Nutrigenomics, Faculty of Pharmacy, Medical University "Prof. Dr. Paraskev Stoyanov "-Varna. 



Катедра: Department of Biochemistry, Molecular Medicine and Nutrigenomics, Faculty of Pharmacy
 УС:
 Е-поща: oskan.tasinov@gmail.com
Телефон: +35952677050

 

Биография

Medical University "Prof. Dr. Paraskev Stoyanov "-Varna, Bulgaria, Specialist – Biochemistry, 01st January 2016

Plovdiv University "Paisii Hilendarski", Plovdiv, Bulgaria, Master of Sciences – Molecular Biology and Biotechnologies, September 2009- March 2011

Sofia University "St. Kliment Ohridski ", Sofia, Bulgaria, Bachelor of Sciences – Molecular Biology, September 2005 – July 2009

Scientific degrees and academic positions:

Medical University "Prof. Dr. Paraskev Stoyanov "-Varna, Bulgaria, PhD on Biochemistry – 23rd March 2015

Academic positions Chief Assist. Prof. – 10th February 2016

Academic positions Assist. Prof. – 01st March 2010 – 10th February 2016

Areas of professional interest:

The subcellular mechanisms of oxidative stress and inflammation; Molecular mechanisms of insulin resistance and diabetes in humans and cell culture models; Analyzing gene expression of proteins, miRNAs and circRNAs as potential predictive and prognostic-diagnostic molecular biomarkers in plasma and biopsies of patients with colorectal carcinoma; Molecular biology, biochemistry, molecular genetics, nutrigenomics, transcriptomics, proteomics.

Membership in scientific organizations:

NuGO – Европейската организация по нутригеномика (since 2010 г.)

FEBS – Federation of European Biochemical Societies (since 2013 г.)

USB – Union of Scientists in Bulgaria, Section of Biochemistry, Biophysics and Molecular Biology (since 2013 г.)

Union of Scientists - Varna (since 2012 г.)

ABDB – Association of Biochemical Departments in Bulgaria (since 2010 г.)  

Specializations and work abroad:

Spain, Santiago de Compostela, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), at the University of Santiago de Compostela, mentored by Prof. Ruben Nogueiras, PhD and Prof. Carlos Dieguez, PhD, 25 January – 25 February 2014

Ireland, Dublin, University College Dublin, comprehensive training "Molecular Nutrition & Regulation of Cardiometabolic Health", 02 June – 04 June 2015

Italy, Napoli, Castellemmare di Stabia, The European Nutrigenomics Organisation (NuGO), post-graduate course, "Nutrigenomics studies in humans: from epidemiology to intervention", 07 – 09 September 2014

Austria, Graz, Medical University of Graz, Institute of Pathology, comprehensive course "Advances in diagnostic molecular pathology", 13 July – 14 July 2015

United Kingdom, Cirencester, Homeopathy at Wellie Level, Abbey Home Farm, "Homeopathy for farmers, vets and scientists" 05 – 10 November 2017

 

Scientific - research activity

Research on the biological effects of medicinal plants, including Sambucus ebulus, Morus nigra, Agrimonia eupatoria in interventions with healthy volunteers and experimental models of oxidative stress and lipopolysaccharide stimulated inflammatory response in cell cultures. Analyzing the molecular mechanisms of the cytoprotective, immunostimulating, anti-inflammatory, antioxidant, insulin-like and antiobesity effects of aqueous and aqueous-alcoholic extracts of medicinal plants. Analyzing and evaluating the biochemical changes and the changes in the transcriptional levels of selected molecular and genetic biomarkers, by applying modern molecular biological methods, such as ELISA, Western blot, qPCR for analysis of gene expression and mutational status (SNP).

Analyzing the gene expression of proteins, miRNA and circRNA, by molecular biological methods, as potential predictive and prognostic-diagnostic molecular biomarkers in plasma and biopsy material of patients with colorectal carcinoma.  

Study of the effect of some cyanotoxins released during the bloom of cyanoprokaryotes on the incidence of cancer in the Bulgarian population. Cytotoxicity assessment of cyanotoxins in cultured human epidermal cell lines.  

Study of the molecular mechanisms of the immunostimulating and cytoprotective actions of a homeopathic monopreparation Ferrum phosforicum D6 in experimental models of murine preadipocyte and macrophage cell lines, in conditions of oxidative stress and inflammatory response.

Optimization of methods for the isolation of intact RNA from cultured human and mouse cell lines, from fresh tissue material, and biopsy material from formalin-fixed, paraffin-embedded tissue for efficient application in subsequent transcriptional analysis. Validation of experimental models in mouse cell cultures and those derived from isolated human peripheral blood mononuclear cells (PBMC). 


Публикации

 

 

Book chapter:

  1. Ivanova D., Nashar M., Kiselova-Kaneva Y., Tasinov O., Vankova D., Nazifova-Tasinova N. The Impact of Human Intervention Studies on the Evaluation of Medicinal Plant Antioxidant and Anti-Inflammatory Activities. In "Human Health and Nutrition: New Research". Ed. S.M. Ostojic. Hauppauge NY, USA, Nova Science Publishers, 2015, 145-166. ISBN:978-1-63482-853-6

 

Scientific conferences:

He participated in 12 national and over 45 international scientific conferences, at 4 of which he was awarded for his presentations.

 

Honours and awards:

  1. "Best Paper Award" for young scientists for poster presentation entitled „Effects of Dwarf elder fruit infusion on NFκB and glutathione metabolism related genes expression in a model of LPS challenged macrophages" in 3rd International Conference on Bio-antioxidants (BIO-ANTIOXIDANTS 2019), 17–21 September 2019, Nessebar, Bulgaria.
  2. Award of Union of Scientist Varna, Bulgaria for young scientist in section "Healthcare and sport" for 2019. 
  3. Jury Award for Best performance by a young scientist in oral presentation on a topic „Relative gene expression of selected miRNAs as possible biomarkers in colorectal cancer stage development", on the conference Modern Molecular-biochemical Markers in Clinical and Experimental Medicine, 31.10-02.11.2018г. Prague, Czech Republic.
  4. Award of Union of Scientists in Bulgaria for Scientific achievements in PhD thesis, Oct, 2015. Title of awarded PhD thesis "Study of antioxidant, antiobesity and antidiabetic action of fruit extracts of Sambucus ebulus in vitro and in vivo"
  5. 13th International Congress of Medical Sciences (ICMS), Sofia, Bulgaria, 8-11 May, 2014. Second place in Preclinic Poster Session with the research "Dwarf elder suppresses LPS induced TNFα and IL-6 gene expression"

    11th International Congress of Medical Sciences (ICMS) Sofia, Bulgaria, 03-06 May, 2012. Second place in Preclinic Poster Session with the research "Sambucus ebulus fruits suppress ethanol induced cytotoxicity and iNOS and COX-2 gene expression in 3T3-L1 mouse preadipocytes"

     

    Research Projects/own research grants (#14 and #18)

    Financed by Bulgarian funds:
  1. 2020 project № 19034. „Plasma levels and expression of leptin and adiponectin to evaluate their applicability as molecular biomarkers for colorectal cancer.",  funded by fund "Science" of Medical University – Varna, 2020. Coordinator   assoc. prof. Yoana Kiselova-Kaneva, PhD. Budget 49999.96lv. – member of the scientific team, as a postdoc.
  2. 2018-2020 project contract № КП-06-ОПР03/18. „Algal toxins – a new risk factor for the quality of life and human health in Bulgaria", funded by National Science Fund of the Ministry of Education and Science – 2018. Coordinator prof. dr. Hristo Gagov, PhD Sofia University "St. Kliment Ohridski". – member of the scientific team, as a postdoc.
  3. 2019-2020 project № 18034 contract № ФН-10/11.02.2019. „Improvement of the infrastructure of the Center for Translation Medicine and Cell Therapy and creation of the first corneal and sclera bank outside Sofia City", funded by fund "Science" of Medical University – Varna, 2018. Coordinator assoc. prof. Yoana Kiselova-Kaneva, PhD. Budget 114 860,21lv. – member of the scientific team, as a postdoc.
  4. 2018-2021 project contract № КП 06-ОПР03/13/18.11.2018. „Study of the adaptogenic potential of Sambucus ebulus fruits for use as a functional food", funded by National Science Fund of the Ministry of Education and Science – 2018. Coordinator assoc. prof. Yoana Kiselova-Kaneva, PhD. Budget 120 000lv. – member of the scientific team, as a postdoc.
  5. project № 18022. „Evaluation of expression levels of selected microRNAs as potential biomarkers in children and adolescents with inflammatory bowel disease", funded by fund "Science" of Medical University – Varna, 2018. Coordinator assoc. prof. dr. Miglena Georgieva, PhD. Budget 6 998,82 lv. – member of the scientific team, as a postdoc.
  6. 2018-2020 project № 18027 – „Lipid nanoparticles - a modern technological approach to include hyperforin with improved chemical stability in topical formulations and accelerated wound healing", funded by fund "Science" of Medical University – Varna, 2018. Coordinator assoc. prof. Velichka Andonova, PhD. Budget 49 845,71lv. – member of the scientific team, as a postdoc.
  7. 2018-2019 project № 18030 – „Tissue and serum expression of Nrf2, NF-KB, HO-1, 4-HNE, and their potential for endothelial dysfunction biomarkers in an experimental model of metabolic syndrome", funded by fund "Science" of Medical University – Varna, 2018. Coordinator prof. dr. Ganka Bekyarova, Dsc. Budget 7000lv. – member of the scientific team, as a postdoc.
  8. 2018-2021 project contract № КП-06-Н23/6/18.12.2018. "Expression profiling of non-coding RNAs in colorectal cancer", funded by National Science Fund of the Ministry of Education and Science – 2018. Coordinator assoc. prof. Maria radanova, PhD. Budget 120 000lv. – member of the scientific team, as a postdoc, work package manager.
  9. 2017-2020 project №Н13/41 contract ДН13/9/15.12.2017. „Cyanocarcinomas – a new potential risk factor for malignancies in Bulgaria?", funded by National Science Fund of the Ministry of Education and Science – 2017. Coordinator prof. Dsc Maya Stoyneva-Gertner. Budget 120 000lv. – member of the scientific team, as a postdoc.
  10. 2018-2020 project №17020, contract № ФМН-41/18.12.2017. „Preparation of an anthocyanin extract of Sambucus ebulus and characterization of its biological activity in relation to the establishment of an adaptogenic potential with a view to its use as a raw material in the production of food and curative substances", funded by fund "Science" of Medical University – Varna, 2017. Coordinator assoc. prof. Yoana Kiselova-Kaneva, PhD budget 7000lv. – member of the scientific team, as a postdoc.
  11. 2018-2020 project №17019, contract № ФМН-53/18.12.2017. „Investigation of single nucleotide polymorphisms in non-coding RNAs in colorectal cancer patients to identify new diagnostic, prognostic and predictive biomarkers for the Bulgarian population", funded by fund "Science" of Medical University – Varna, 2017. Coordinator assoc. prof. Maria radanova, PhD, budget 49 928,79lv. – member of the scientific team, as a postdoc.
  12. 2018-2019 project №17015, contract № ФМН-42/18.12.2017. „Study of the effects of sulfur containing mineral waters on human sulphur metabolism", funded by fund "Science" of Medical University – Varna, 2017. Coordinator assoc. prof. Milka Asparuhova Nachar, PhD, budget 7000lv. – member of the scientific team, as a postdoc.

 

  1. 2017-2019 project № Н01/42/03.09.2016. " Study of the molecular mechanisms of action of sulfur containing mineral waters from the Varna basin on the human metabolism with a view to their use as a therapeutic-drinking water", funded by National Science Fund of the Ministry of Education and Science, competition for funding of fundamental research – 2016. Coordinator Prof. Dsc Diana Ivanova. – member of the scientific team, as a postdoc.

 

  1. 2016-2017 project №16020, contract № НИ-3/5.12.2016. „Exploration of gene expression of some poorly studied proteins and micro RNAs in view of their application as molecular markers in colorectal cancer patients", funded by fund "Science" of Medical University – Varna, 2016. Coordinator Chief Assist. Prof. Oskan Tasinov, PhD. – member of the scientific team, as a postdoc.

 

  1. 2015-2016 project №15005, contract № ФМН-87/21.12.2015. – Infrastructure project "Establishment of infrastructure for controlled concentration of biologically active substances from natural products", funded by fund "Science" of Medical University – Varna, 2015. Coordinator prof. Bistra Galunska, PhD. – member of the scientific team, as a PhD student.
  2. 2015-2016г project №14013/18.12.2014, contract №1/18.12.2015 „Exploring the therapeutic potential of black mulberry wood as a resource for the pharmaceutical industry", funded by fund "Science" of Medical University – Varna, 2014. Coordinator prof. Dsc Diana Ivanova. – member of the scientific team.

Financed from foreign for Bulgaria funds:

  1. 2012-2015 project КВВЕ.2011.2.2-02 „New technologies and tools and their potential application to nutrition research", funded by the EU's 7th Framework Program. – member of the scientific team.
  2. 25.01-25.02.2014 project: „Investigation of the effect of Sambucus ebulus L. extract on energy marker levels associated with lipogenesis and insulin sensitivity". Financed by a scholarship of the "Science and Business" project BG051PO001-3.3.05-0001 of the Ministry of Education and Science, Republic of Bulgaria, under the program "Human Resources Development", co-financed by the European Social Fund of the European Union. One-month research exchange in the Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), at the University of Santiago de Compostela, Spain, mentored by Prof. Ruben Nogueiras, PhD and Prof. Carlos Dieguez, PhD. – scholarship holder.
  3. 2013-2014 project: BG051PO001-3.3.06-028 "Increasing the research potential and career development opportunities in the field of medicine, health and biotechnology" financed by the program "Human Resources" – scholarship holder. 


Book chapter:

  1. Ivanova D., Nashar M., Kiselova-Kaneva Y., Tasinov O., Vankova D., Nazifova-Tasinova N. The Impact of Human Intervention Studies on the Evaluation of Medicinal Plant Antioxidant and Anti-Inflammatory Activities. In "Human Health and Nutrition: New Research". Ed. S.M. Ostojic. Hauppauge NY, USA, Nova Science Publishers, 2015, 145-166. ISBN:978-1-63482-853-6

 

Scientific conferences:

He participated in 12 national and over 45 international scientific conferences, at 4 of which he was awarded for his presentations.

 

Honours and awards:

  1. "Best Paper Award" for young scientists for poster presentation entitled „Effects of Dwarf elder fruit infusion on NFκB and glutathione metabolism related genes expression in a model of LPS challenged macrophages" in 3rd International Conference on Bio-antioxidants (BIO-ANTIOXIDANTS 2019), 17–21 September 2019, Nessebar, Bulgaria.
  2. Award of Union of Scientist Varna, Bulgaria for young scientist in section "Healthcare and sport" for 2019. 
  3. Jury Award for Best performance by a young scientist in oral presentation on a topic „Relative gene expression of selected miRNAs as possible biomarkers in colorectal cancer stage development", on the conference Modern Molecular-biochemical Markers in Clinical and Experimental Medicine, 31.10-02.11.2018г. Prague, Czech Republic.
  4. Award of Union of Scientists in Bulgaria for Scientific achievements in PhD thesis, Oct, 2015. Title of awarded PhD thesis "Study of antioxidant, antiobesity and antidiabetic action of fruit extracts of Sambucus ebulus in vitro and in vivo"
  5. 13th International Congress of Medical Sciences (ICMS), Sofia, Bulgaria, 8-11 May, 2014. Second place in Preclinic Poster Session with the research "Dwarf elder suppresses LPS induced TNFα and IL-6 gene expression"

    11th International Congress of Medical Sciences (ICMS) Sofia, Bulgaria, 03-06 May, 2012. Second place in Preclinic Poster Session with the research "Sambucus ebulus fruits suppress ethanol induced cytotoxicity and iNOS and COX-2 gene expression in 3T3-L1 mouse preadipocytes"

     

    Research Projects/own research grants (#14 and #18)

    Financed by Bulgarian funds:
  1. 2020 project № 19034. „Plasma levels and expression of leptin and adiponectin to evaluate their applicability as molecular biomarkers for colorectal cancer.",  funded by fund "Science" of Medical University – Varna, 2020. Coordinator   assoc. prof. Yoana Kiselova-Kaneva, PhD. Budget 49999.96lv. – member of the scientific team, as a postdoc.
  2. 2018-2020 project contract № КП-06-ОПР03/18. „Algal toxins – a new risk factor for the quality of life and human health in Bulgaria", funded by National Science Fund of the Ministry of Education and Science – 2018. Coordinator prof. dr. Hristo Gagov, PhD Sofia University "St. Kliment Ohridski". – member of the scientific team, as a postdoc.
  3. 2019-2020 project № 18034 contract № ФН-10/11.02.2019. „Improvement of the infrastructure of the Center for Translation Medicine and Cell Therapy and creation of the first corneal and sclera bank outside Sofia City", funded by fund "Science" of Medical University – Varna, 2018. Coordinator assoc. prof. Yoana Kiselova-Kaneva, PhD. Budget 114 860,21lv. – member of the scientific team, as a postdoc.
  4. 2018-2021 project contract № КП 06-ОПР03/13/18.11.2018. „Study of the adaptogenic potential of Sambucus ebulus fruits for use as a functional food", funded by National Science Fund of the Ministry of Education and Science – 2018. Coordinator assoc. prof. Yoana Kiselova-Kaneva, PhD. Budget 120 000lv. – member of the scientific team, as a postdoc.
  5. project № 18022. „Evaluation of expression levels of selected microRNAs as potential biomarkers in children and adolescents with inflammatory bowel disease", funded by fund "Science" of Medical University – Varna, 2018. Coordinator assoc. prof. dr. Miglena Georgieva, PhD. Budget 6 998,82 lv. – member of the scientific team, as a postdoc.
  6. 2018-2020 project № 18027 – „Lipid nanoparticles - a modern technological approach to include hyperforin with improved chemical stability in topical formulations and accelerated wound healing", funded by fund "Science" of Medical University – Varna, 2018. Coordinator assoc. prof. Velichka Andonova, PhD. Budget 49 845,71lv. – member of the scientific team, as a postdoc.
  7. 2018-2019 project № 18030 – „Tissue and serum expression of Nrf2, NF-KB, HO-1, 4-HNE, and their potential for endothelial dysfunction biomarkers in an experimental model of metabolic syndrome", funded by fund "Science" of Medical University – Varna, 2018. Coordinator prof. dr. Ganka Bekyarova, Dsc. Budget 7000lv. – member of the scientific team, as a postdoc.
  8. 2018-2021 project contract № КП-06-Н23/6/18.12.2018. "Expression profiling of non-coding RNAs in colorectal cancer", funded by National Science Fund of the Ministry of Education and Science – 2018. Coordinator assoc. prof. Maria radanova, PhD. Budget 120 000lv. – member of the scientific team, as a postdoc, work package manager.
  9. 2017-2020 project №Н13/41 contract ДН13/9/15.12.2017. „Cyanocarcinomas – a new potential risk factor for malignancies in Bulgaria?", funded by National Science Fund of the Ministry of Education and Science – 2017. Coordinator prof. Dsc Maya Stoyneva-Gertner. Budget 120 000lv. – member of the scientific team, as a postdoc.
  10. 2018-2020 project №17020, contract № ФМН-41/18.12.2017. „Preparation of an anthocyanin extract of Sambucus ebulus and characterization of its biological activity in relation to the establishment of an adaptogenic potential with a view to its use as a raw material in the production of food and curative substances", funded by fund "Science" of Medical University – Varna, 2017. Coordinator assoc. prof. Yoana Kiselova-Kaneva, PhD budget 7000lv. – member of the scientific team, as a postdoc.
  11. 2018-2020 project №17019, contract № ФМН-53/18.12.2017. „Investigation of single nucleotide polymorphisms in non-coding RNAs in colorectal cancer patients to identify new diagnostic, prognostic and predictive biomarkers for the Bulgarian population", funded by fund "Science" of Medical University – Varna, 2017. Coordinator assoc. prof. Maria radanova, PhD, budget 49 928,79lv. – member of the scientific team, as a postdoc.
  12. 2018-2019 project №17015, contract № ФМН-42/18.12.2017. „Study of the effects of sulfur containing mineral waters on human sulphur metabolism", funded by fund "Science" of Medical University – Varna, 2017. Coordinator assoc. prof. Milka Asparuhova Nachar, PhD, budget 7000lv. – member of the scientific team, as a postdoc.

 

  1. 2017-2019 project № Н01/42/03.09.2016. " Study of the molecular mechanisms of action of sulfur containing mineral waters from the Varna basin on the human metabolism with a view to their use as a therapeutic-drinking water", funded by National Science Fund of the Ministry of Education and Science, competition for funding of fundamental research – 2016. Coordinator Prof. Dsc Diana Ivanova. – member of the scientific team, as a postdoc.

 

  1. 2016-2017 project №16020, contract № НИ-3/5.12.2016. „Exploration of gene expression of some poorly studied proteins and micro RNAs in view of their application as molecular markers in colorectal cancer patients", funded by fund "Science" of Medical University – Varna, 2016. Coordinator Chief Assist. Prof. Oskan Tasinov, PhD. – member of the scientific team, as a postdoc.

 

  1. 2015-2016 project №15005, contract № ФМН-87/21.12.2015. – Infrastructure project "Establishment of infrastructure for controlled concentration of biologically active substances from natural products", funded by fund "Science" of Medical University – Varna, 2015. Coordinator prof. Bistra Galunska, PhD. – member of the scientific team, as a PhD student.
  2. 2015-2016г project №14013/18.12.2014, contract №1/18.12.2015 „Exploring the therapeutic potential of black mulberry wood as a resource for the pharmaceutical industry", funded by fund "Science" of Medical University – Varna, 2014. Coordinator prof. Dsc Diana Ivanova. – member of the scientific team.

Financed from foreign for Bulgaria funds:

  1. 2012-2015 project КВВЕ.2011.2.2-02 „New technologies and tools and their potential application to nutrition research", funded by the EU's 7th Framework Program. – member of the scientific team.
  2. 25.01-25.02.2014 project: „Investigation of the effect of Sambucus ebulus L. extract on energy marker levels associated with lipogenesis and insulin sensitivity". Financed by a scholarship of the "Science and Business" project BG051PO001-3.3.05-0001 of the Ministry of Education and Science, Republic of Bulgaria, under the program "Human Resources Development", co-financed by the European Social Fund of the European Union. One-month research exchange in the Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), at the University of Santiago de Compostela, Spain, mentored by Prof. Ruben Nogueiras, PhD and Prof. Carlos Dieguez, PhD. – scholarship holder.
  3. 2013-2014 project: BG051PO001-3.3.06-028 "Increasing the research potential and career development opportunities in the field of medicine, health and biotechnology" financed by the program "Human Resources" – scholarship holder. 


Член на

​Е (NuGO) ets.