Medical University "Prof. Dr. Paraskev Stoyanov "-Varna, Bulgaria, Specialist – Biochemistry, 01st January 2016
Plovdiv University "Paisii Hilendarski", Plovdiv, Bulgaria, Master of Sciences – Molecular Biology and Biotechnologies, September 2009- March 2011
Sofia University "St. Kliment Ohridski ", Sofia, Bulgaria, Bachelor of Sciences – Molecular Biology, September 2005 – July 2009
Scientific degrees and academic positions:
Medical University "Prof. Dr. Paraskev Stoyanov "-Varna, Bulgaria, PhD on Biochemistry – 23rd March 2015
Academic positions Chief Assist. Prof. – 10th February 2016
Academic positions Assist. Prof. – 01st March 2010 – 10th February 2016
Areas of professional interest:
The subcellular mechanisms of oxidative stress and inflammation; Molecular mechanisms of insulin resistance and diabetes in humans and cell culture models; Analyzing gene expression of proteins, miRNAs and circRNAs as potential predictive and prognostic-diagnostic molecular biomarkers in plasma and biopsies of patients with colorectal carcinoma; Molecular biology, biochemistry, molecular genetics, nutrigenomics, transcriptomics, proteomics.
Membership in scientific organizations:
NuGO – Европейската организация по нутригеномика (since 2010 г.)
FEBS – Federation of European Biochemical Societies (since 2013 г.)
USB – Union of Scientists in Bulgaria, Section of Biochemistry, Biophysics and Molecular Biology (since 2013 г.)
Union of Scientists - Varna (since 2012 г.)
ABDB – Association of Biochemical Departments in Bulgaria (since 2010 г.)
Specializations and work abroad:
Spain, Santiago de Compostela, Center for Research in Molecular Medicine and Chronic Diseases (CIMUS), at the University of Santiago de Compostela, mentored by Prof. Ruben Nogueiras, PhD and Prof. Carlos Dieguez, PhD, 25 January – 25 February 2014
Ireland, Dublin, University College Dublin, comprehensive training "Molecular Nutrition & Regulation of Cardiometabolic Health", 02 June – 04 June 2015
Italy, Napoli, Castellemmare di Stabia, The European Nutrigenomics Organisation (NuGO), post-graduate course, "Nutrigenomics studies in humans: from epidemiology to intervention", 07 – 09 September 2014
Austria, Graz, Medical University of Graz, Institute of Pathology, comprehensive course "Advances in diagnostic molecular pathology", 13 July – 14 July 2015
United Kingdom, Cirencester, Homeopathy at Wellie Level, Abbey Home Farm, "Homeopathy for farmers, vets and scientists" 05 – 10 November 2017
Scientific - research activity
Research on the biological effects of medicinal plants, including Sambucus ebulus, Morus nigra, Agrimonia eupatoria in interventions with healthy volunteers and experimental models of oxidative stress and lipopolysaccharide stimulated inflammatory response in cell cultures. Analyzing the molecular mechanisms of the cytoprotective, immunostimulating, anti-inflammatory, antioxidant, insulin-like and antiobesity effects of aqueous and aqueous-alcoholic extracts of medicinal plants. Analyzing and evaluating the biochemical changes and the changes in the transcriptional levels of selected molecular and genetic biomarkers, by applying modern molecular biological methods, such as ELISA, Western blot, qPCR for analysis of gene expression and mutational status (SNP).
Analyzing the gene expression of proteins, miRNA and circRNA, by molecular biological methods, as potential predictive and prognostic-diagnostic molecular biomarkers in plasma and biopsy material of patients with colorectal carcinoma.
Study of the effect of some cyanotoxins released during the bloom of cyanoprokaryotes on the incidence of cancer in the Bulgarian population. Cytotoxicity assessment of cyanotoxins in cultured human epidermal cell lines.
Study of the molecular mechanisms of the immunostimulating and cytoprotective actions of a homeopathic monopreparation Ferrum phosforicum D6 in experimental models of murine preadipocyte and macrophage cell lines, in conditions of oxidative stress and inflammatory response.
Optimization of methods for the isolation of intact RNA from cultured human and mouse cell lines, from fresh tissue material, and biopsy material from formalin-fixed, paraffin-embedded tissue for efficient application in subsequent transcriptional analysis. Validation of experimental models in mouse cell cultures and those derived from isolated human peripheral blood mononuclear cells (PBMC).